IN VITRO CALLUS REGENERATION OF BRASSICA SPP THROUGH ANTHER CULTURE (Short Communication)

Abstract

Brassica is an important oil-yielding crop under Brassicaceae family. Brassica is the third most important vegetable oil crop in the world, after palm and soyabean oil. But it takes a long time to develop new Brassica cultivars. Haploid production of Brassica spp. through anther culture proved to be an important approach of tissue culture. The purpose of this study was to carry out a suitable protocol for happloid production of Brassica spp. and observe genotypic variation for callus induction. Three replications were used containing different concentration of 2, 4-D, BAP, Kiniten and NAA. The highest percentage of callus induction (56.2%) was found in CM 800 within minimum days (18 days) and the nature of callus was compact. It indicates totipotency capacity of cell was also influenced by radiation. Future study was necessary to shoot and root induction process for successful speed breeding program.

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